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Ahvaz Jundishapur University of Medical Sciences

Algorithm-Based Fetal Gender Determination Using X and Y Mini-Short Tandem Repeats at Early Gestational Ages

Aghanoori, Mohamad-Reza and Mohammadzadeh Shahriary, Ghazaleh and Khorrami, Mehdi and Sayadi, Mehrab and Yazdani, Yasaman and Yazdani, Saman and Akhond, Mohamad Reza and Goodarzi, Hamedreza (2016) Algorithm-Based Fetal Gender Determination Using X and Y Mini-Short Tandem Repeats at Early Gestational Ages. Jentashapir Journal of Health Research, In Pre (InPres). ISSN 2345-4067

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Official URL: http://doi.org/10.17795/jjhr.29365

Abstract

Background: Detection of fetal DNA in maternal blood has been examined by many research groups for a few years; thereby, scientists have a shorter way to take to approach prenatal diagnosis of abnormal pregnancies. The Y chromosome sequences have recently become the most common applicable indices for fetal sex determination. Objectives: We conducted an algorithmic X and Y mini-Short Tandem Repeats (STRs) genotyping method that could solve the problem of false negative (no detection of Y sequences) results of previous methods. Patients and Methods: Blood samples were obtained from 106 pregnant women and their spouses. Conventional PCR amplified 19 mini-Short Tandem Repeats (STRs) and three non-STR markers, which were subsequently genotyped by the means of Polyacrylamide gel electrophoresis (PAGE). Results: Sensitivity and specificity of the mini-STR genotyping method for fetal DNA detection were calculated (95.9% and 98%, respectively) with a confidence interval of 95%. In addition, sensitivity and informativeness were computed for each of the single mini-STR markers in our conventional PCR method. We also introduced the minimum number of mini-STRs needed to reach maximum validity for fetal gender determination in clinical settings. Conclusions: Algorithm-based mini-STR genotyping method significantly increases the reliability (sensitivity and specificity) of gender determination using free fetal DNA and could be applied in prenatal clinical testing.

Item Type: Article
Keywords: Free Fetal DNA, Sex Determination, X-Y Algorithm, Mini-STR
Subjects: R Medicine > R Medicine (General)
Depositing User: آقای بهرام سوسنی غریب وند
Date Deposited: 21 Nov 2016 10:49
Last Modified: 21 Nov 2016 10:49
URI: http://eprints.ajums.ac.ir/id/eprint/2689

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