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Ahvaz Jundishapur University of Medical Sciences

Effects of Crocin on Spatial Memory Impairment Induced by Hyoscine and Its Effects on BDNF, CREB, and p-CREB Protein and mRNA Levels in Rat Hippocampus

Behravanfar, Nima and Abnous, Khalil and Razavi, Bibi Marjan and Hosseinzadeh, Hossein (2017) Effects of Crocin on Spatial Memory Impairment Induced by Hyoscine and Its Effects on BDNF, CREB, and p-CREB Protein and mRNA Levels in Rat Hippocampus. Jundishapur Journal of Natural Pharmaceutical Products, In Pre (In Pre). ISSN 1735-7780

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Official URL: http://doi.org/10.5812/jjnpp.64315

Abstract

Background: Crocus sativus L., (Iridaceace), known as saffron, is used in traditional medicine for different purposes such asmemory improvment. Crocin is one of the pharmacologically active constituents responsible for many medicinal properties of saffron. Objectives: In this study, the effects of crocin, an active saffron constituent, on hyoscine inducedmemory impairment and its effect onprotein andmRNAtranscript levels of CREB (cAMP response element binding protein), BDNF(brain-derived neurotrophic factor), and phospho-CREB (p-CREB) were investigated in rat hippocampus. Methods: Rats were intraperitoneally treated with crocin (10, 20, and 40 mg/kg), normal saline (1 mL/kg), and rivastigmine (1.5 mg/kg) 30 minutes prior to hyoscine (1 mg/kg) for five days. The effects were studied on spacial learning and memory using Morris water maze. Protein and transcript levels of CREB and BDNF were analyzed using western blotting and qRT PCR methods, respectively. Results: Five days treatment with crocin (20 and 40 mg/kg) and rivastigmine significantly increased the presence of animals in target quadrant in probe trial on the 8th day compared to hyoscine. Crocin (20 mg/Kg) increased significantly BDNF and CREB protein and transcript levels compared to hyoscine. Conclusions: Crocin improved memory impairment induced by hyoscine through reduction of the latency to find the platform in the probe trial in Morris water maze test. Crocin improved memory probably due to the increase of BDNF and CREB protein and transcript levels.

Item Type: Article
Keywords: Crocin, BDNF, CREB, p-CREB, MorrisWater Maze Test, Spatial Memory
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: آقای بهرام سوسنی غریب وند
Date Deposited: 16 Jan 2018 05:36
Last Modified: 16 Jan 2018 05:36
URI: http://eprints.ajums.ac.ir/id/eprint/11443

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