Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ahvaz Jundishapur University of Medical Sciences

Purification of blood coagulation factor V activator from the venom of Iranian Vipera lebetina

Amozegari, Zohreh and Zare Mirakabadi, Abbas and Noorbehbahani, Mozhgan (2013) Purification of blood coagulation factor V activator from the venom of Iranian Vipera lebetina. Jundishapur Scientific Medical Journal. ISSN 2252-0619

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Abstract

Background and Objective: The venom of many Viperidae snake appear to contain proteins that affect blood coagulation. The aim of this study was to identify and isolate a blood coagulation factor V activator present in the venom from Vipera lebetina. Material and Methods: Factor V activator was purified from 200 mg of crude venom by three steps of chromatorgraphy which included: gel filtration on sephadex G100, Ion exchange chromatography on DEAE and cellulose and affinity chromatography on heparin agarose. The effect of factor V activator on human factor V was studied by measuring the amidiolytic activity of the produced thrombin by activated factor V (Va). Results: The results of SDS – PAGE identified an activator of factor V as a single protein band with a molecular weight of 29 KDa. This compound was converted, in the presence of calcium ions, to active factor Va. It also had arginine esterase activity toward substrate BAEE ( -benzoyl arginine ethyester) and a weak amidase activity on S-2222 (benzoyl- le-glu-Gly-Arg-p- nitroanilide). Conclusion: The results of this study showed that the isolated factor is a specific activator on factor V. ►Please cite this paper as: Purification of blood coagulation factor V activator from the venom of Iranian Vipera lebetina. Amoozegari Z, Zare Mirak A, Noorbehbahani M. Jundishapur Sci Med J 2013;12(1):21-32

Item Type: Article
Keywords: Keywords: factor V activity, snake venom, Vipera lebetina
Subjects: Q Science > QP Physiology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: آقای بهرام سوسنی غریب وند
Date Deposited: 21 Apr 2018 07:10
Last Modified: 13 Aug 2018 04:09
URI: http://eprints.ajums.ac.ir/id/eprint/12691

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