Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ahvaz Jundishapur University of Medical Sciences

Ginsenoside Rb1 stimulates adiponectin signaling in C2C12 muscle cells through up-regulation of AdipoR1 and AdipoR2 proteins

Tabandeh, M. R. and Jafari, H. and Hosseini, S. A. and Hashemitabar, M. (2015) Ginsenoside Rb1 stimulates adiponectin signaling in C2C12 muscle cells through up-regulation of AdipoR1 and AdipoR2 proteins. Pharmaceutical Biology, 53 (1). pp. 125-132. ISSN 13880209 (ISSN)

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Abstract

Context: Rb1 ginsenoside, the key element of ginseng root, is widely used as an herbal therapeutic drug in diabetic patients. Various hypoglycemic mechanisms have been described for Rb1; however, to date, there has been no report on the effect of Rb1 on adiponectin signaling. Objectives: The current study was performed to establish the effects of ginsenoside Rb1 on the gene expression of AdipoR1 and AdipoR2 and their correlation to GLUT4 translocation in C2C12 myocytes. Materials and methods: C2C12 myotubes were incubated with various concentrations of Rb1 (0.001-100 μM) for different incubation times (1-12 h). Real time PCR and western blot analyses were performed to investigate the expression changes of adiponectin receptors (AdRs) and GLUT4 translocation, respectively. Gene silencing of AdipoR1 using specific siRNA was used to determine whether inhibition of AdipoR1 would reduce Rb1-induced GLUT-4 translocation in C2C12 cells. Results: Rb1 significantly stimulated basal AdRs expression levels in a time and dose-dependent manner; the maximal effect was attained at a concentration of 100 μM and a time of 3 h (p < 0.05). In muscle cells, Rb1 increased GLUT4 translocations to the cell surface, which was correlated with increasing the adiponectin receptors gene expression. Rb1-induced GLUT4 translocation was inhibited by the silencing of AdipoR1 mRNA. Discussion and conclusion: These results suggest that ginsenoside Rb1 promote translocations of GLUT4 by activating the adiponectin signaling pathway. The results can be helpful in understanding the novel antidiabetic mechanism of Rb1 ginsenoside and gain further support for its use as an antidiabetic drug. © 2015 Informa Healthcare USA, Inc. All rights reserved.

Item Type: Article
Keywords: Antidiabetic effect Molecular mechanism Muscle cell adiponectin adiponectin receptor 1 adiponectin receptor 2 ginsenoside Rb 1 glucose transporter 4 messenger RNA small interfering RNA adiponectin receptor adiponectin receptor 1, mouse adiponectin receptor 2, mouse Adipoq protein, mouse antidiabetic agent ginsenoside Slc2a4 protein, mouse Article cell surface cellular distribution concentration response controlled study gene expression gene silencing signal transduction upregulation animal cell line cell membrane drug effects genetics metabolism mouse myoblast protein transport real time polymerase chain reaction Western blotting Panax ginseng Animals Blotting, Western Ginsenosides Glucose Transporter Type 4 Hypoglycemic Agents Mice Myoblasts Real-Time Polymerase Chain Reaction Receptors, Adiponectin Up-Regulation
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine, Health and Life Sciences
Depositing User: مهندس مهدی شریفی
Last Modified: 01 Jun 2018 11:32
URI: http://eprints.ajums.ac.ir/id/eprint/16516

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