Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ahvaz Jundishapur University of Medical Sciences

Aflatoxin M-1 detoxification from infected milk using Fe3O4 nanoparticles attached to specific aptamer

Jouni, F. J. and Zafari, J. and Abdolmaleki, P. and Vazini, H. and Ghandi, L. and Satari, M. (2018) Aflatoxin M-1 detoxification from infected milk using Fe3O4 nanoparticles attached to specific aptamer. Journal of Nanostructure in Chemistry, 8 (1). pp. 13-22. ISSN 2008-9244

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Official URL: <Go to ISI>://WOS:000429488000002


Aflatoxins are a kind of mycotoxins that are mostly produced by a group of molds such as Aspergillus flavus and Aspergillus parasiticus. The studies on different parts of Iran showed that AFM(1) infection of milk is higher than European Union's standard. In this study, the specific aptamer with the carboxylic group at the end of 5' and Fe3O4 nanoparticles with amine groups was synthesized. Morphological and structural qualities of Fe3O4 were determined by the Fourier-transform infrared spectrograph (FTIR), dynamic light scattering, scanning electron microscope, and X-ray diffraction devices. The specificity of the aptamer to AFM(1) was investigated in adjacent of AFM(1) and aflatoxin B-1. Then, aptamers were attached to nanoparticles to improve synthetic qualities and to ease of its detachment. The attachment was approved by FTIR method. The complex (Fe3O4-APT) was then added to infected milk and after the proper time was detached from the milk using a magnet. The remained amount of AFM(1) was attained in milk using high-performance liquid chromatography. Our result showed that this method for aflatoxin detoxification is much more effective than conventional methods based on recognition of AFM(1) and their concentration in infected milk. This method is more applicable, faster, and cheaper highlighting its application in milk factories. GRAPHICS .

Item Type: Article
Keywords: aflatoxin aptamer fe3o4 nanoparticle detoxification mycotoxin on photoelectrochemical immunoassay magnetic nanoparticles electrochemical immunoassay corn samples m1 signal b-1 contamination biomolecules dots
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine, Health and Life Sciences
Depositing User: مهندس مهدی شریفی
Last Modified: 01 Jun 2018 13:08

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