Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ahvaz Jundishapur University of Medical Sciences

Development of a radiolabeled glucagon compound for imaging

Jalilian, A. R. and Jouiaei, M. and Doroudi, A. R. and Bolourinovin, F. and Garousi, J. (2010) Development of a radiolabeled glucagon compound for imaging. Nukleonika, 55 (2). pp. 219-224. ISSN 0029-5922

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Official URL: <Go to ISI>://WOS:000279161300015

Abstract

In order to develop a possible Ga-labeled glucagon (GCG) compound for imaging studies, biosynthetic glucagon (GCG) was labeled with (67)Ga-gallium chloride after conjugation with freshly prepared diethylenetriaminepentaacetic acid dianhydride (ccDTPA). After solid phase purification of the radiolabeled hormone, high performance liquid chromatography (HPLC) and instant thin-layer chromatography (ITLC) showed a radiochemical purity around 95% in optimized conditions (specific activity = 296-370 GBq/mM; labeling efficiency 85%). Preliminary in vivo studies (ID.g(-1)%) in male wild-type rats showed heart: muscle, liver: muscle, lung: muscle and stomach: muscle ratios to be 5.53, 2.9, 7.56, 3.69, 3.2 (in 5 min), respectively while after 2 h liver:blood, lung:blood and spleen :blood ratios were 14.21, 16.86 and 7.8, respectively. The data suggests 5 min post injection, the tracer is accumulated in GCGR rich tissues which is in agreement with biodistribution studies and reported GCG receptors (GCGRs). The results of the present study can possibly offer a candidate for non-invasive imaging of glucagon receptor related diseased and malignancies such as glucagonoma.

Item Type: Article
Keywords: glucagons radiolabeling biodistribution ga-67 identification hepatocytes antibody receptor complex rats
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine, Health and Life Sciences
Depositing User: مهندس مهدی شریفی
Last Modified: 01 Jun 2018 13:13
URI: http://eprints.ajums.ac.ir/id/eprint/18683

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