Repository of Research and Investigative Information

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Ahvaz Jundishapur University of Medical Sciences

Detection of IgM and IgG anti-Toxoplasma antibodies in renal transplant recipients using ELFA, ELISA and ISAGA methods: Comparison of pre-and posttransplantation status

Gharavi, M. J. and Jalali, S. and Khademvatan, S. and Heydari, S. (2011) Detection of IgM and IgG anti-Toxoplasma antibodies in renal transplant recipients using ELFA, ELISA and ISAGA methods: Comparison of pre-and posttransplantation status. Annals of Tropical Medicine and Parasitology, 105 (5). pp. 367-371. ISSN 00034983 (ISSN)

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Abstract

In the transplant recipient patients receive immunosuppressive therapy, the possibility of reactivation of the old infection or acquisition of infection from a donor's tissue increases. In this study, IgMand IgG anti-Toxoplasma immunoglobulins seroconversion in renal transplant recipients (RTRs) have been evaluated before and after transplantation. This is a prospective cohort study on a total of 102 RTRs. Two serum samples were obtained from each patient. The first was taken before administration of any immunosuppressive drugs such as corticosteroids and the second was taken 3 months after transplantation. The IgM and IgG anti-Toxoplasma antibodies were assayed by enzymelinked flourescence assay (ELFA) and enzyme-linked immunosorbent assay (ELISA) techniques. IgM/ immunosorbent agglutination assay (ISAGA) method has also been used. All RTRs were tested for toxoplasmosis before and after transplantation. ELFA identified 65 (63.7) pretransplantation samples as IgGz and did not detect any positive IgM samples. However, IgM was detected in three (2.9) post-transplantation samples by this method. Forty-nine (48) pre-transplantation samples were reported IgGz by ELISA and no IgM positive sample was identified by this method. ELISA has detected two (1.9) IgM-positive reactions in post-transplantation samples. By IgM/ISAGA method, we have detected no IgM positive reactions in pretransplantation samples, whereas 3 months later (second sampling) IgM antibody was detected in 3 (2.9) cases. Secondary toxoplasmosis infection was observed in 30 cases per 1000 RTRs, which indicates that screening for toxoplasmosis infection should be performed in developed countries for these patients. On the other hand, as the risk of re-active toxoplasmosis infection exists in developing nations, they should consider the necessary preventive measures to control this condition. © W. S. Maney & Son Ltd 2011.

Item Type: Article
Keywords: corticosteroid immunoglobulin G immunoglobulin M adolescent adult aged agglutination test antibody detection antigen antibody reaction article cohort analysis enzyme linked immunosorbent assay female graft recipient human immunofluorescence test kidney graft major clinical study male prospective study serum Toxoplasma toxoplasmosis transplantation Antibodies, Anti-Idiotypic Antibodies, Protozoan Cohort Studies Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique Humans Immunoenzyme Techniques Immunosorbent Techniques Immunotherapy Kidney Transplantation Middle Aged Prospective Studies Sensitivity and Specificity Young Adult
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine, Health and Life Sciences
Depositing User: مهندس مهدی شریفی
Last Modified: 01 Jun 2018 13:13
URI: http://eprints.ajums.ac.ir/id/eprint/18895

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