Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ahvaz Jundishapur University of Medical Sciences

Crocin attenuates cisplatin-induced renal oxidative stress in rats

Naghizadeh, B. and Mansouri, S. M. T. and Mashhadian, N. V. (2010) Crocin attenuates cisplatin-induced renal oxidative stress in rats. Food and Chemical Toxicology, 48 (10). pp. 2650-2655. ISSN 02786915 (ISSN)

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Abstract

Reactive oxygen species (ROS) and oxidative damage are the most important factors in cisplatin-induced acute renal failure. This study examined the protective effects of crocin against cisplatin-induced renal oxidative stress in rat. Animals were divided into five groups (n=6). Group 1 received normal saline (2ml/day, i.p.). Group 2 received a single dose of cisplatin (5mg/kg, i.p.). Groups 3-5 received crocin (100, 200, and 400mg/kg, i.p., respectively) for four consecutive days beginning 1-h before a single dose of cisplatin (5mg/kg) on day 1. On day 5, blood samples were drawn and kidneys were removed for histopathological, biochemical and RT-PCR examinations. Twenty four hours urinary chemistries were measured. Blood urea and creatinine and urinary glucose and protein concentrations in crocin-treated groups were significantly lower compared to the cisplatin-treated group. Histopathological studies showed massive damage in the S3 segment of proximal tubules in cisplatin-treated group but not in crocin-treated groups. Crocin treatment resulted in a significant reduction in malondialdehyde (MDA) concentration and produced a significant elevation in total thiol and glutathione peroxidase concentrations. There was a significant elevation in the mRNA expression of glutathione peroxidase in crocin-treated groups. The results suggest that crocin attenuates cisplatin-induced renal oxidative stress in rats. © 2010 Elsevier Ltd.

Item Type: Article
Keywords: Anti-oxidant Cisplatin Crocin Renal oxidative stress creatinine glucose glutathione peroxidase malonaldehyde messenger RNA sodium chloride thiol urea animal experiment animal model animal tissue antioxidant activity article controlled study creatinine blood level glucose urine level kidney injury kidney proximal tubule male nephrotoxicity nonhuman oxidative stress protein expression rat renal protection urea blood level Animals Antineoplastic Agents Blood Urea Nitrogen Carotenoids Glycosuria Kidney Malondialdehyde Proteinuria Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA Sulfhydryl Compounds Thiobarbituric Acid Reactive Substances Animalia Rattus
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine, Health and Life Sciences
Depositing User: مهندس مهدی شریفی
Last Modified: 01 Jun 2018 13:13
URI: http://eprints.ajums.ac.ir/id/eprint/19047

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