Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ahvaz Jundishapur University of Medical Sciences

In vitro effect of Naringin and Quercetin on LDL oxidation via their influence in copper binding to LDL

Ghaffari, M. A. and Ghiasvand, T. (2007) In vitro effect of Naringin and Quercetin on LDL oxidation via their influence in copper binding to LDL. Iranian Journal of Diabetes and Lipid Disorders, 7 (1). ISSN 17267544 (ISSN)

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Abstract

Background: The oxidative modification of low density lipoprotein (LDL) may play an important role in atherogenesis. Mechanism of LDL oxidation and factors that determine its susceptibility to oxidation is unknown. Copper is account as an attributing factor in LDL oxidation atherosclerotic lesions. The binding of copper ions to LDL is usually thought to be a prerequisite for LDL oxidation by copper. The aim of study was to investigate the effect of Naringin and Quercetin on copper bound to LDL and furthermore effect of this binding on the susceptibility of LDL to oxidative modification. Methods: LDL was isolated from EDTA-plasma by ultracentrifugation using a discontinuous gradient. The oxidizability of LDL was measured by thiobarbitoric acid reactive substances (TBARS) after addition of CuSO4 in absence and/or presence of Naringin and Quercetin. Finally effect of Naringin and Quercetin on formation of LDL-copper complex was studied by gel filtration. Results: This study showed that Naringin suppresses formation of TBARS and LDL-copper complex, whereas Quercetin enhances formation of TBARS and LDL-copper complex. Conclusion: Results revealed that Naringin with inhibition of binding of Copper to LDL may decrease the susceptibility of LDL oxidation in counter to this ion and thus may have a role in prevention of atherosclerosis. But Quercetin with stimulation of binding of copper to LDL may increase the susceptibility of LDL oxidation to this ion and thus may have a role in progression atherosclerosis.

Item Type: Article
Keywords: Copper LDL Naringin Quercetin aurantiin copper sulfate edetic acid low density lipoprotein thiobarbituric acid reactive substance article atherosclerosis chemical binding disease course gel filtration in vitro study oxidation ultracentrifugation
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine, Health and Life Sciences
Depositing User: مهندس مهدی شریفی
Last Modified: 01 Jun 2018 13:14
URI: http://eprints.ajums.ac.ir/id/eprint/19501

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