Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ahvaz Jundishapur University of Medical Sciences

Isolation and identification of 1,2-dihydroxy-9,10-anthraquinone (Alizarin) from the roots of Maddar plant (Rubia tinctorum)

Ashnagar, A. and Naseri, N. G. and Zadeh, A. S. (2007) Isolation and identification of 1,2-dihydroxy-9,10-anthraquinone (Alizarin) from the roots of Maddar plant (Rubia tinctorum). Biosciences Biotechnology Research Asia, 4 (1). pp. 19-22. ISSN 09731245 (ISSN)

Full text not available from this repository.

Abstract

The roots of madder (Rubia tinctorum) are source of anthraquinone dyes with alizarin being the main component. Free alizarin (I) is present in madder root in only small quantities, most of it is present as its glycoside i.e. ruberythric acid(III) On the basis of the importance of alizarin, in this research, it was isolated, purified and its structure was confirmed by various spectra. Maceration of the powdered madder roots in various polar and non-polar solvents, as well as Soxhlet extraction resulted in the formation of a reddish solid material (5.2 and 14.2 yield, respectively). Successive TLC and column chromatography of the solid material on silica gel with methanol as the mobile phase, gave three fractions with Rf = 0.21, 0.48 and 0.68. The fraction with Rf =0.68 was the major one. 1HNMR, 13CNMR, IR, UV and Mass spectra of this fraction were taken. On the basis of the results obtained from the spectra, the chemical structure of alizarin was determined and confirmed.

Item Type: Article
Keywords: 1,2-dihydroxy-9,10-anthracenedione 1,2-dihydroxy-9,10-anthraquinone Alizarin Madder roots Ruberythric acid Rubia tinctorum methanol plant extract plant glycoside purpurin rubia tinctorum extract silica gel unclassified drug article carbon nuclear magnetic resonance chemical analysis chemical composition chemical structure column chromatography infrared spectroscopy isolation procedure mass spectrometry nonhuman plant root proton nuclear magnetic resonance purification Rubia solvent extraction Soxhlet extraction thin layer chromatography
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine, Health and Life Sciences
Depositing User: مهندس مهدی شریفی
Last Modified: 01 Jun 2018 13:14
URI: http://eprints.ajums.ac.ir/id/eprint/19535

Actions (login required)

View Item View Item