Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ahvaz Jundishapur University of Medical Sciences

Application of restriction enzyme analysis technique based on 65kDa heat shock protein gene for fingerprinting and differentiation of Mycobacterium tuberculosis clinical strains isolated from tuberculosis patients in Ahwaz, Iran

Khosravi, A. D. and Hashemi, A. (2007) Application of restriction enzyme analysis technique based on 65kDa heat shock protein gene for fingerprinting and differentiation of Mycobacterium tuberculosis clinical strains isolated from tuberculosis patients in Ahwaz, Iran. Pakistan Journal of Medical Sciences, 23 (2). pp. 216-219. ISSN 1682024X (ISSN)

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Abstract

Objective: Application of identification methodology of restriction enzyme analysis (REA) for fingerprinting of the expanded population of Mycobacterium tuberculosis (MTB) isolates. Methodology: A total of 150 clinical isolates from patients referred to TB reference laboratory, Public Health Centre, Ahwaz, Iran, were identified as MTB by using conventional culture and biochemical tests from January to December 2004. The PCR-REA method uses a PCR step based on amplification of a 439 bp fragment of hsp65 gene involving genus specific primers and restriction enzyme analysis by digestion of products with HaeIII & BstEII enzymes were employed. Results: The identical restriction patterns similar to MTB reference strains equal to 160/145/72bp fragments for Hae III and 250/120/82bp fragments for Bst EII digests were seen in 145 isolates (96.6). The diverse patterns were observed for five isolates in Hae III digest as 180/100/80 bp, 194/72 bp and 160/145 bp representing the possible intra-species variation within studied MTB strains , while their Bst EII digestion patterns showed no variation. Conclusions: The PCR-REA technique revealed three different new patterns for Hae III digest. However to verify that they are indeed MTB isolates, a sequence-based analysis of the exceptional isolates should be performed.

Item Type: Article
Keywords: 65 heat shock protein gene M. tuberculosis PCR-REA Restriction enzyme heat shock protein 65 adult aged article bacterial strain bacterium culture bacterium isolate biochemistry controlled study DNA fingerprinting DNA sequence female genetic variability human intraspecific variation Iran laboratory lung tuberculosis male Mycobacterium tuberculosis nonhuman polymerase chain reaction restriction mapping tuberculosis
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine, Health and Life Sciences
Depositing User: مهندس مهدی شریفی
Last Modified: 01 Jun 2018 13:14
URI: http://eprints.ajums.ac.ir/id/eprint/19552

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