Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ahvaz Jundishapur University of Medical Sciences

The expression of signal regulatory protein-α in normal and osteoarthritic human articular cartilage and its involvement in chondrocyte mechano-transduction response

Orazizadeh, M. and Salter, D. M. (2007) The expression of signal regulatory protein-α in normal and osteoarthritic human articular cartilage and its involvement in chondrocyte mechano-transduction response. Iranian biomedical journal, 11 (2). pp. 119-124. ISSN 1028852X (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background: Signal regulatory proteins (SIRP) belong to immunoglobulin super family (IgSF) and relate to integrin signaling cascades. It has been shown that SIRPα is expressed in a variety of cells including myeloid cells and neurons. In the present study the expression of this IgSF member in articular chondrocytes was investigated. Methods: Using a panel of anti-SIRPα antibodies, immunohistochemistry, Western-blotting and electrophysiology methods, expression of SIRPα and its role in chondrocyte mechano-transduction were assessed. Results: No identifiable positive signal was obtained by using immunohistochemistry methods on frozen and paraffin sections. SIRPα is expressed by both normal and osteoarthritis cultured chondrocytes. The electrophysiological response of chondrocytes in the presence of SE7C2 mAb was significantly inhibited whereas; SE5A5 did not show any modification in this response. Conclusions: It seems likely that SIRPα could be associated with other proteins such as integrins, CD47 and ion channels, which contribute to the electrophysiological response of human articular chondrocytes. In any case, this study has provided a specific functional role for SIRPα in chondrocyte mechano-transduction.

Item Type: Article
Keywords: Cartilage Chondrocyte Osteoarthritis (OA) Signal regulatory proteins (SIRP) monoclonal antibody monoclonal antibody se5a5 monoclonal antibody se7c2 paraffin protein antibody regulator protein signal regulatory protein alpha signal regulatory protein alpha antibody unclassified drug article articular cartilage cartilage cell cell culture cell membrane depolarization cell stimulation controlled study electrophysiology frozen section human human cell human tissue immunohistochemistry mechanical stimulation mechanotransduction osteoarthritis protein expression protein function Western blotting Antigens, Differentiation Blotting, Western Cartilage, Articular Cell Culture Techniques Chondrocytes Humans Mechanotransduction, Cellular Receptors, Immunologic
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine, Health and Life Sciences
Depositing User: مهندس مهدی شریفی
Last Modified: 01 Jun 2018 13:14
URI: http://eprints.ajums.ac.ir/id/eprint/19557

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