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Repository of Research and Investigative Information

Ahvaz Jundishapur University of Medical Sciences

Verapamil and Rifampin Effect on P-Glycoprotein Expression in Hepatocellular Carcinoma

Jalali, Amir and Ghasemian, Sepideh and Najafzadeh, Hossein and Galehdari, Hamid and Seifi, Masoudreza and Zangene, Fateme and Dehdardargahi, Shaiesteh (2014) Verapamil and Rifampin Effect on P-Glycoprotein Expression in Hepatocellular Carcinoma. Jundishapur Journal of Natural Pharmaceutical Products, 9 (4). pp. 1-4. ISSN 2228-7876

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Abstract

Background: High expression of p-glycoprotein (P-gp) has been associated with a poor prognosis in patients with hepatocellular carcinoma (HCC). It is likely that P-gp overexpression is responsible for multidrug resistance in HCC. Objectives: The aim of this study was to elucidate the effect of potent carcinogen nitrosamine with and without verapamil and rifampin drugs on P-gp expression at the mRNA level in HCC. Materials and Methods: Four groups of rats (n = 5) were selected with different treatments and one group as control. mRNA concentration changes were monitored using quantitative PCR (QPCR). Results: A significant difference was found between verapamil treated group and the control regarding the mRNA level. The mdr1a mRNA was significantly decreased in the verapamil group (P ≤ 0.001). Rifampin administrated group had a decreased level of the mdr1a mRNA compared to the control group (P ≤ 0.006). No significant changes were observed in HCC induced rats regarding the mdr1a mRNA level when treated with verapamil and rifampin. An enhanced expression of the mdr1a gene was found In the HCC induced animals when treated with drugs. Conclusions: Verapamil and rifampin were found specific and effective against P-gp expression in HCC. In conclusion, treatment efficacy of most anticancer drugs is increased in combination with verapamil and rifampin against most advanced HCC.

Item Type: Article
Keywords: P-Glycoprotein; Hepatocellular Carcinoma; Rifampin; Verapamil; Marla Gene Copyright
Subjects: R Medicine > R Medicine (General)
Depositing User: آقای بهرام سوسنی غریب وند
Date Deposited: 27 Jun 2015 23:36
Last Modified: 10 Feb 2016 06:59
URI: http://eprints.ajums.ac.ir/id/eprint/2016

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