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Ahvaz Jundishapur University of Medical Sciences

Study of CAPE Effect on Apoptosis Induction in AGS Human Gastric Cancer Cell Line

Amini-Sarteshnizi, Nematallah and Teimori, Hossein and Beshkar, Pezhman and Amini-Sarteshnizi, Roghayeh and Nikoukar, Morteza (2016) Study of CAPE Effect on Apoptosis Induction in AGS Human Gastric Cancer Cell Line. Jundishapur Journal of Natural Pharmaceutical Products, 11 (1). ISSN 1735-7780

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Official URL: http://doi.org/10.17795/jjnpp-22534

Abstract

Background:Propolis is a natural product of bee and caffeic acid phenethyl ester (CAPE) is a pharmacologically important product of propolis. Objectives: The aim of this study was to investigate the effect of CAPE on apoptosis induction in AGS human gastric cancer cells. Materials and Methods: The cytotoxic effects of CAPE at different concentrations were investigated on AGS cells viability after 24 hours treatment by MTT assay. To measure the effect of CAPE on apoptosis induction, AGS cells were treated with CAPE for 24 hours and investigated by FITC Annexin V/PI staining using flow cytometry. Results: CAPE prevented growth and proliferation of AGS human gastric cancer cell line in a concentration-dependent manner with an IC50 of approximately 60 µM by a 24-hour treatment. Also CAPE caused increased induction of apoptosis in AGS cells from 1.37 % in control cells to 21.76 % in treated cells with 30 µM CAPE. Conclusions: CAPE prevents growth and proliferation of AGS human gastric cancer cell line through inducing programmed cell death in AGS cells. Therefore, CAPE could be helpful for developing chemotherapeutic agents or as an adjuvant for human gastric cancer treatment.

Item Type: Article
Keywords: Caffeic Acid Phenethyl Ester; Cell Survival; Apoptosis; Cancer
Subjects: R Medicine > R Medicine (General)
Depositing User: آقای بهرام سوسنی غریب وند
Date Deposited: 23 Jan 2016 06:20
Last Modified: 07 Feb 2016 08:24
URI: http://eprints.ajums.ac.ir/id/eprint/2179

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