Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ahvaz Jundishapur University of Medical Sciences

Measurement of Hyaluronidase Enzyme Activity in Venom of Iranian Vipera Lebetina

Amozegari, Zohreh and ابیض, سمانه and نوربهبهانی, مژگان (2016) Measurement of Hyaluronidase Enzyme Activity in Venom of Iranian Vipera Lebetina. Jundishapur Scientific Medical Journal, 15 (3). pp. 363-370. ISSN 2252-0619

[img]
Preview
Text
af_6935733363659539323433252423692763445443_jmsiSZHMRSDCB.pdf

Download (799kB) | Preview

Abstract

Background and Objective: Snake venoms comprise complex mixtures of enzymatic and non-enzymatic proteins and small organic compounds. Hyaluronidase is a constant factor in the venom of the snake and is known as a spreading factor. This factor facilitate spread of toxins that harm into the blood circulation system. In this paper, we describe the determination of hyaluronidase activity in Iranian Vipera lebetina venom. Subjects and Methods: Hyaluronidase activity was assayed turbidometrically on hyaluronic acid. Turbidity reducing activity was expressed as a percentage of the hydrolysed hyaluronate, taking the absorbance of a tube as 100% in which no enzyme was added. Bovin testis hyaluronidase was used as standard. Results: The optima PH and temperature for hyaluronidase maximum activity was 6 and 37 °C respectively. Conclusion: In conclusion the Iranian Vipera lebetina venom possessed considerable hyaluronidase activity.

Item Type: Article
Keywords: Vipera lebetina, Venom, Hyaluronidase
Subjects: R Medicine > R Medicine (General)
Depositing User: آقای بهرام سوسنی غریب وند
Date Deposited: 12 Dec 2016 06:16
Last Modified: 12 Dec 2016 06:16
URI: http://eprints.ajums.ac.ir/id/eprint/2844

Actions (login required)

View Item View Item