Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ahvaz Jundishapur University of Medical Sciences

Bioassay Guided Fractionation of an Anti-Methicillin-Resistant Staphylococcus aureus Flavonoid From Bromus inermis Leyss Inflorescences

Aliahmadi, Atousa and Mirzajani, Fateme and Ghassempour, Alireza and Sonboli, Ali (2014) Bioassay Guided Fractionation of an Anti-Methicillin-Resistant Staphylococcus aureus Flavonoid From Bromus inermis Leyss Inflorescences. Jundishapur Journal of Microbiology, 7 (12). pp. 1-4. ISSN 2008-4161

[img]
Preview
Text
jjm-7-12-e12739.pdf - Published Version

Download (389kB) | Preview

Abstract

Background: Plants are considered as promising sources of new antibacterial agents as well as bioassay guided fractionation. Objectives: In the present work, the antibacterial properties, especially against methicillin-resistant Staphylococcus aureus (MRSA), of Bromus inermis inflorescence was studied, using the bioassay guided fractionation as well as the bio-autographic method. Materials and Methods: The plant organic extract was prepared via maceration in methanol, followed by the fractionation using n-hexane. The extracts were subjected for minimum inhibitory concentrations (MICs) against some human pathogenic bacteria via standard broth micro-dilution assay. Thereafter, a bio-autographical method was applied using the high performance thin layer chromatography (HPTLC) coupled with agar overlay assays for the primary characterization and identification of bioactive substance (s). Results: Through the bioassay guided fractionation method, the greatest antibacterial activities were related to the n-hexane extract. It was also revealed that the effective anti-MRSA agent of the assessed plant was a relatively polar substance with an MIC value of about 8 μg/mL against the tested MRSA strain (in comparison with the MIC value of 32 μg/mL for chloramphenicol). Conclusions: As a result of the full range UV-Vis scanning of the responsible band in the HPTLC experiments (200-700 nm), the flavonoid was the most imaginable natural compound.

Item Type: Article
Keywords: Chemical Fractionation; Methicillin-Resistant Staphylococcus aureus, Flavonoid, Anti-bacterial Agents
Subjects: R Medicine > R Medicine (General)
Depositing User: آقای بهرام سوسنی غریب وند
Date Deposited: 01 Jun 2015 08:54
Last Modified: 10 Feb 2016 06:26
URI: http://eprints.ajums.ac.ir/id/eprint/490

Actions (login required)

View Item View Item